【类型】期刊
【题名】Pathogens Identification and Control of Edible Roses Leaf Spot Diseases in Guizhou
【作者】 莫维弟
【关键词】 炭疽病 Field Control 药剂筛选 Leaf Black Spot Edible Roses 黑斑病 田间防治 Fungicide Screening Anthracnose 食用玫瑰 生物防治 Biological Control
【期刊名】Bioprocess
【摘要】In order to identify the pathogenicspecies of edible roses leaf spot diseases in Guizhou Botanical Garden andHuishui County, Guizhou Province, China, the samples of leaf spot diseases werecollected. By using single spore isolation method and pathogenicitydetermination, combined with morphological analysis combined with ITS, GADPH, ACT, CHS-1 gens, the taxonomic statusof its pathogenic bacteria was determined. On this basis, through platedilution and plate confrontation method, combined with physiological andbiochemical experiments and 16SrRNA and gyrA gens, screening and identification ofantagonistic bacteria on edible rose, then combined with the mycelial growthrate method to screen the chemical agents with better control effect on roseleaf spot, commercial microbial preparation Lvkangwei, Myclobutanil 12% EC + Dithane65% WP, Oxadixyl mancozeb 64% WP and GUHS97 were used for field control effectexperiments. The results showed that: 1) The pathogen causing leaf black spotwas confirmed to be Marssinina rosae, and the pathogen causinganthracnose was confirmed to be Colletotrichum boninense; 2) In thisstudy, a strain GUHS97 was screened from the healthy rose leaves in the heavilyinfected field and was identified as Bacillus amyloliquefaciens; 3)Indoor toxicity test showed that 9 fungicides had a certain inhibitory effecton C. boninense. Theinhibition effects of zhongshengmycin 3% WP, difenoconazole 10% WDG,pyraclostrobin 25% SC and metalaxyl∙fludioxonil 62.5% SC were higher with theEC50 values of 0.8440 mg∙L−1、1.3854 mg∙L−1、1.8667 mg∙L−1 and 3.5945 mg∙L−1,respectively, followed by ningnanmycin 8% AS and bromothalonil 25% EC, with theEC50 values of 18.9624 mg∙L−1 and 25.4813 mg∙L−1,respectively. Pyraclostrobin 25% SC and bromothalonil 25% EC at the mixtureratio of 1:7, 1:8 and 1:10 showed obvious synergistic effect; 4) Furthermore,GUHS97 exhibited 59.12% control over leaf spot in field experiments and wasmore effective than other treatments including fungicide application. Overall,these results provide a scientific basis for the identification of edible roseleaf diseases and their control.
【年份】2022
【ISSN】2164-5566
【页码】63-80
【期号】2
【作者单位】贵州大学农学院，贵州 贵阳 贵州省植物园，贵州 贵阳 北京市植物保护站，北京 遵义师范学院资源与环境学院，贵州 遵义 贵州大学烟草学院，贵州 贵阳 贵州大学农学院，贵州 贵阳；贵州省农业科学院，贵州 贵阳
【期刊卷】12
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