描述:A new bioassay method of residual film in glass tube was developed for the study of Diaphorina citri (Kuwayama) adult. The sensitivity of 4 field populations of D. citri to 6 insecticides was evaluated. The results indicated that the D. citri was susceptible to thiacloprid, thiamethoxam and chlorpyrifos with the resistance ratios in the range of (0.57-3.07)-fold. However, Lingchuan population in Guangxi Province developed moderate level of resistance to imidacloprid, with the resistance ratio of 18.90-fold. Meanwhile, Lingchuan population developed 9.72-fold resistance to lambda-cyhalothrin. Moreover, Lingchuan and Yongfu populations in Guangxi Province had developed low resistance levels to the novel insecticide sulfoxaflor, and the resistance ratios were over 5-fold. These results were in accordance with the reported documents about the control efficacy in field. It is helpful to set up a standard method to monitor the sensitivity dynamic of D. citri.
描述:Pydiflumetofen is a novel succinate dehydrogenase inhibitor (SDHI), which shows excellent inhibitory activity to many plant pathogens, such as Fusarium graminearum and Sclerotinia sclerotiorum. In this study, the sensitivity to pydiflumetofen of Passalora fulva strains collected from 8 cities in Shandong Province were tested by using mycelial growth rate method. In addition, the control efficacy of pydiflumetofen to tomato leaf mold were also investigated in field. The results showed that pydiflumetofen had strong inhibitory activity on three developmental stages of P. fulva, and the inhibitory activities on spore gemination and germ tube elongation were higher than that on mycelial growth. Inhibition rate of pydiflumetofen to spore gemination and germ tube elongation of P. fulva was over 50% at 0.10 μg/mL. The EC50 values frequency distribution of 103 P. fulva isolates to pydiflumetofen on mycelial growth was a unimodal curve and could be regarded as the baseline sensitivity. The EC50 values ranged from 0.04 to 1.74 μg/mL, with a mean of (0.67 ± 0.41) μg/mL. In field trials of two years, after the last application at the dose of 200 g a.i./hm², the control efficacy of pydiflumetofen was above 80%, which was significantly higher than that of thiophanate-methyl (540 g a.i./hm² ) and fluopyram (150 g a.i./hm² ). This study provides data support for monitoring the resistance of tomato leaf mold to pydiflumetofen and for efficient controlling tomato leaf mold.
描述:In order to discover efficient, low toxicity and environmentally friendly pesticide lead compounds, the inhibitory effects of five sterol biosynthesis inhibitors (SBIs) antifungal drugs (econazole, fluconazole, voriconazole, ketoconazole and miconazole) against seven plant pathogens were determined by mycelial growth rate method. After the preliminarily screening, effective fungicides were further tested in greenhouse and in field. The results showed that voriconazole had the highest fungicidal activities against the seven plant pathogens, and its EC50 values were lower than 0.349 mg/L. Miconazole exhibited potent fungicidal activities against Fusahum graminearum, Alternaria kikuchiana, Fusarium oxysporum and Glomerella cingulata. Econazole showed strong fungicidal activities against A. kikuchiana and F. oxysporum, ketoconazole displayed excellent fungicidal activities against Magnaporthe oryzae. Both their EC50 value were higher than that of difenoconazole against the corresponding plant pathogens. In greenhouse, voriconazole and fluconazole showed excellent inhibitory effects against wheat powdery mildew with the control efficiencies of 98.26% and 89.11%, respectively, at the dosage of 37.5 mg/L, which were higher than that of the commercial fungicide triadimenol. At the dosage of 150 mg/L, econazole showed strong inhibitory effect against rice sheath blight with the control efficiency of 86.14%. In the field trial, the control efficiency of fluconazole against wheat stripe rust was 98.42%, and the control efficacy of econazole against rice sheath blight was 75.21% at the dose of 240 g a.i./hm2. Fluconazole, voriconazole and econazole exhibited potent fungicidal activities against the tested plant pathogens, which can be used as lead compounds for agricultural fungicides.
描述:In order to clarify the insecticide resistance status in the field populations of the small brown planthopper (Laodelphax striatellus (Fallén)) from Henan Province, the resistances to 12 commonly used insecticides in seven field populations of L. striatellus collected from Xinxiang, Puyang, Kaifeng, Zhumadian, and Xinyang in Henan Province of China during 2020 to 2021 were detected by rice seedling dipping method. The results showed that the field populations of L. striatellus have developed medium-level resistance to buprofezin (resistance ratio, RR=14.9-91.1). Furthermore, the field populations produced low to medium-level resistance to pymetrozine (RR=6.91-16.7) and chlorpyrifos (RR=8.48-70.0) and were susceptible to medium-level resistance to triazophos (RR=1.29-11.1). In addition, all field populations of L. striatellus were susceptible or low-level resistance to thiamethoxam (RR=0.95-5.19) and lambda-cyhalothrin (RR=3.31-7.24), and relatively susceptible to imidacloprid (RR=0.89-3.92), acetamiprid (RR=1.11-2.33), nitenpyram (RR=0.16-0.64), dinotefuran (RR=1.87- 3.86), isoprocarb (RR=0.47-1.37) and flonicamid (RR=1.63-4.33), respectively. The results shed light on the sustainable control of L. striatellus field population and scientific use of insecticides in Henan Province.
描述:Flupyradifurone is a new neonicotinoid insecticide developed by Bayer CropScience. In order to explore the application prospects of flupyradifurone on rice planthopper, the resistance of ten field populations of Nilaparvata lugens from seven provinces in China and eight field populations of Sogatella furcifera from five provinces in China to flupyradifurone were evaluated using the riceseedling dip method. The results showed that the populations of N. lugens in those ten areas showed low to moderate resistance [resistance ratio (RR)=6.1−17.4], among which the populations from Nanchang in Jiangxi, Xinyang in Henan, Liuan in Anhui, and Hangzhou in Zhejiang exhibited moderate resistance (RR=10.1−17.4). In contrast, all field populations of S. furcifera were relatively sensitive to flupyradifurone (RR=1.1−3.6) except the population (RR=6.3) in Xiaogan. This study investigated the cuRRent resistance status of field populations of N. lugens and S. furcifera to flupyradifurone, and provids a theoretical basis for the scientific application of flupyradifurone in the control of rice planthopper.
描述:Penoxsulam is a new acetolactate synthase inhibitor (ALS) herbicide developed by Dow AgroSciences. In order to explore the sensitivity of Echinochloa spp. to penoxsulam on rice field, whole-plant bioassay experiments were conducted to evaluate the resistance level of 70 field populations of Echinochloa spp. from main rice production in seven provinces of China. The results showed that 50.0% of the 70 populations had evolved high resistance, 47.1% of the populations was moderate resistance, and only 2.9% of the populations exhibited low resistance. None populations were sensitive to penoxsulam. More than 60% of the populations had evolved high resistance from Hubei, Anhui, Ningxia and Heilongjiang Province. The populations of high level resistance was more than 25% in other three province. More than 60% of the population with moderate level resistance were from Hunan, Jiangxi and Jiangsu Province, and the populations of 20.0%-40.0% were also with moderate level resistance in others 4 provinces. The 10.0% and 12.5% of population were with low level resistance in Hubei and Heilongjiang Province, while other 5 provinces had no low level resistant populations. This work investigated the evaluation and monitoring of the field resistance of populations of Echinochloa spp. to penoxsulam, which provided a theoretical basis for the scientific application of penoxsulam to control Echinochloa spp. in the main rice cultivation area.
描述:On the basis of briefly describing the research progress of humic acid in agriculture in recent years and the preparation principle of humic acid fertilizer, the research progress of field trials of humic acid green low ・ carb on fertilizer is summarized ・ Introduced that humic acid has greatly improved the utilization rate of chemical fertilizers, that is, reducing the loss of fertilizer nutrients and promoting the absorption of nutrients・ The rational application of different types of humic acid fertilizers is conducive to impro・ ving crop yield and quality, for example, humic acid phosphate fertilizer treatment increases corn grain yield by 4.5% to 13.6%; the addition of humate ammonium can increase oil sunflower yield by 23・ 98%; the application of humic acid compound fertilizer increased tomato yield by 16. 4% and cucumber yield by 10. 9%; the add让ion of active humic acid compound fertilizer increased the soluble sugar content of sweet potato by 20. 45% and the soluble protein content by 41. 86%, so the economic benefit was also improved.
描述:To study the new materials for the control of Nilaparvata lugens, sodium alginate and peptone were used as water phase, rosin + sodium alginate film was prepared by adding oil phase composed of rape oil and rosin, and optimizing the ratio of oil phase to water phase. The physicochemical properties and performance indexes such as pH value, viscosity, solid content, film formation time and resistance to rain water erosion were determined, and the indoor inhibition activity and field control efficacy of rosin + sodium alginate film on Nilaparvata lugens were determined by rice straw spray method. The results showed that when the mass fraction of sodium alginate, peptone, rosin and rapeseed oil was 3%, 8%, 12.5% and 10%, respectively, the drawing degree, viscosity and ductility of rosin + sodium alginate film reached the optimal value, showing weak acidity, the pH value was between 6.34 and 6.91, the viscosity was 108 -114 Pa.s, and the solid content was 38.62%. Indoor inhibition activity showed that after being treated with 50 times diluted film for 3 days, the body weight of Nilaparvata lugens decreased significantly, the weight loss rate was as high as 92.78%, and the corrected mortality rate was only 20%. After being treated with 100 times diluted film for 7 d, the corrected mortality of Nilaparvata lugens was 59.26%. The results of field control experiment showed that the control efficacy of 50 times diluted film on Nilaparvata lugens was similar to that of 20% isoprocarb EC diluted 1 500 times, and the control efficacy on Nilaparvata lugens after 7 days were 64.21% and 64.04%, respectively. This study shows that rosin + sodium alginate film has a good control efficacy on Nilaparvata lugens and has potential application value, which provides a new idea for the control of diseases and insect pests in the field.