描述:In order to determine the best fertilization amount and fertilization formula of Momordica charantia in Haikou city, the “2+X” field experiment of fertilization effect was carried out on the laterite soil of Dongshan Town, Haikou City in 2019. The results showed that the yield increase effect was obvious and the yield increase rate up to 12.7%. The yield of the optimized fertilization area was 1628.8 kg/667m2, which was 183.3 kg/667m2 higher than that of the conventional fertilization area. The order of the yield from high to low was optimized fertilization area > conventional fertilization area > 130% optimized nitrogen area > 70% optimized nitrogen area > no nitrogen area. The quadratic equation of nitrogen application rate and yield was established by Excel. The optimal N application rate was 15.91 kg/667m2, and the best economic yield was 1532.28 kg/667m2. The results showed that the optimized fertilization scheme was feasible in summer cultivation of M. charantia.
描述:In order to identify the pathogenicspecies of edible roses leaf spot diseases in Guizhou Botanical Garden andHuishui County, Guizhou Province, China, the samples of leaf spot diseases werecollected. By using single spore isolation method and pathogenicitydetermination, combined with morphological analysis combined with ITS, GADPH, ACT, CHS-1 gens, the taxonomic statusof its pathogenic bacteria was determined. On this basis, through platedilution and plate confrontation method, combined with physiological andbiochemical experiments and 16SrRNA and gyrA gens, screening and identification ofantagonistic bacteria on edible rose, then combined with the mycelial growthrate method to screen the chemical agents with better control effect on roseleaf spot, commercial microbial preparation Lvkangwei, Myclobutanil 12% EC + Dithane65% WP, Oxadixyl mancozeb 64% WP and GUHS97 were used for field control effectexperiments. The results showed that: 1) The pathogen causing leaf black spotwas confirmed to be Marssinina rosae, and the pathogen causinganthracnose was confirmed to be Colletotrichum boninense; 2) In thisstudy, a strain GUHS97 was screened from the healthy rose leaves in the heavilyinfected field and was identified as Bacillus amyloliquefaciens; 3)Indoor toxicity test showed that 9 fungicides had a certain inhibitory effecton C. boninense. Theinhibition effects of zhongshengmycin 3% WP, difenoconazole 10% WDG,pyraclostrobin 25% SC and metalaxyl∙fludioxonil 62.5% SC were higher with theEC50 values of 0.8440 mg∙L−1、1.3854 mg∙L−1、1.8667 mg∙L−1 and 3.5945 mg∙L−1,respectively, followed by ningnanmycin 8% AS and bromothalonil 25% EC, with theEC50 values of 18.9624 mg∙L−1 and 25.4813 mg∙L−1,respectively. Pyraclostrobin 25% SC and bromothalonil 25% EC at the mixtureratio of 1:7, 1:8 and 1:10 showed obvious synergistic effect; 4) Furthermore,GUHS97 exhibited 59.12% control over leaf spot in field experiments and wasmore effective than other treatments including fungicide application. Overall,these results provide a scientific basis for the identification of edible roseleaf diseases and their control.
描述:In order to improve the inhibition activity and light stability of the protein extract TraT2A from the fermentation broth of Trichoderma atroviride T2 strain, the ultraviolet protective agents (ascorbic acid, humic acid) and the auxiliary agent (IE-08, SP-4821A and SP-4821B) were screened, the inhibition condition of TraT2A for Alternaria alternata were optimized through the colony growth rate test, and the field control efficacy on lily leaf spot disease was determined. The results showed that TraT2A had a good inhibitory effect on A.alternata at 200.00 mg/mL and the inhibitory rate was 72.12%. With the prolongation of the UV illumination time, the inhibition activity of TraT2A gradually decreases. The addition of 5.00 mg/mL humic acid had an excellent UV protection effect on TraT2A and its inhibition rate (69.01%) increased by 39.64% compared with that without a UV protection agent (29.37%). The addition of 0.17 mg/mL adjuvant of SP-4821A increased the inhibition activity of TraT2A most significantly and the inhibition rate (95.13%) increased by 25.97% compared with the TraT2A control (69.16%). According to the optimized formula, 200 g TraT2A (the mass concentration was 200.00 mg/mL), 5 g humic acid (the mass concentration was 5.00 mg/mL), 0.17 g SP-4821A (the mass concentration was 0.17 mg/mL) and 794.83 mL sterilized water was mixed and the field control efficacy of the mixture was tested on lily leaf spot disease, the result showed that its field control efficacy was 80.37%. The research results provide a theoretical basis for the further development of TraT2A preparations for the control of lily diseases.
描述:At present, there are few studies on the influence of the cleaning parameters of soybean harvester on the cleaning indexes in China. In order to change the current situation of soybean combine harvester, due to the lack of theoretical guidance for the parameter adjustment of the corresponding cleaning device, the cleaning parameter adjustment is not timely and accurate, resulting in high loss rate and high impurity content of soybean harvester. In this study, the multi parameter adjustable and measurable cleaning system was used to optimize the cleaning parameters of soybean harvester in field test. The influence rules of the five cleaning parameters on the two cleaning indexes were analyzed, and the best cleaning parameter combination was found. The field verification test of the best cleaning parameter combination of soybean harvester was completed. Based on the analysis of the structure of cleaning system and the principle of cleaning operation of soybean harvester, the importance of the operating parameters and operating speed of cleaning device to the cleaning indexes of soybean combine harvester was obtained. The operation speed, opening of chaffer screen, damper opening, fan speed and crank speed of shale shaker were used to optimize the five cleaning parameters in field experiments. Cleaning loss rate and impurity rate were used to optimize the two cleaning indexes of field experiments. Through the analysis of the influence of cleaning device on the indexes of soybean combine harvester, the cleaning loss rate and impurity rate were determined as cleaning indexes, and in the field experiment, the cleaning loss of samples were collected in the form of binding cleaning loss receiving bag at the back of cleaning room to optimize the parameters of cleaning loss in the field experiment. Response surface test design and data analysis are completed by using design expert software. The contribution rate and response effect of each parameter on the two cleaning indexes were analyzed by the contribution rate method and response surface diagram. The results showed that the order of influence of cleaning parameters on cleaning loss rate was crank speed of shale shaker, fan speed, operation speed, damper opening, opening of chaffer screen. The order of influence of cleaning parameters on the impurity rate was opening of chaffer screen, damper opening, fan speed, operation speed, crank speed of shale shaker; The results showed that when the cleaning loss rate was small and the impurity rate was small and the feeding amount was large, the optimal cleaning parameters were operation speed was 6 km/h, opening of chaffer screen was 32 mm, damper opening was 17°, fan speed was 1 310 r/min and crank speed of shale shaker was 410 r/min. At this time, the cleaning loss rate was 0.25%, the impurity rate was 0.61%, and the relative error with the optimized value of the model was 0.250% and 0.113%, respectively. Compared with the cleaning indexes of the field experiment of soybean combine harvester under the common cleaning parameters, the cleaning loss rate was reduced by 0.05%, and the impurity rate was reduced by 2.09%. The results provide a theoretical basis for the setting and adjustment of cleaning parameters and the research and development of self-adaptive cleaning system.
描述:八氢番茄红素脱氢酶(PDS)和ζ-胡萝卜素脱氢酶(ZDS)是类胡萝卜素生物合成途径中的关键酶,其活性受到抑制可导致类胡萝卜素的生物合成受阻,植物出现白化现象,并最终死亡。N-(4-氟苄基)-6-甲基吡啶酰胺(编号:ZI-22)是本课题组通过随机合成筛选出的一个作用于PDS和ZDS的6-甲基吡啶酰胺类专利化合物,具有较好的除草活性和进一步开发利用的潜力。本论文研究了ZI-22的室内除草活性及混配增效、防治玉米田杂草田间药效试验、在土壤中的残留消解动态及抗性风险评价等内容,为其商品化开发和应用技术上提供必要的理论依据。ZI-22室内除草活性及混配增效研究。采用盆栽法,以马齿苋、马唐、牛筋草、苘麻、稗草、狗尾草为供试杂草,ZI-22为供试药剂,进行茎叶喷雾处理。结果显示ZI-22对阔叶杂草的活性要优于禾本科杂草,最敏感的是马齿苋,其ED50值为59.50 g a.i./hm~2;ZI-22与氨唑草酮按照1:9的比例混配后,混剂对禾本科杂草稗草、狗尾草、马唐、牛筋草的除草活性均具有明显的增效作用,同时对玉米表现出较好的安全性。ZI-22与氨唑草酮混用苗后茎叶喷雾防治玉米田杂草的药效试验。结果表明:ZI-22与氨唑草酮混用后对玉米田常见杂草表现出良好的除草活性,能够防治玉米田绝大多数杂草,当混剂施用量为150 g a.i./hm~2时,药后30 d的杂草鲜重防效为85.59%,可作为推荐剂量使用。ZI-22和氨唑草酮在玉米田间土壤中的淋溶性及残留消解动态研究。以关中黄土为试验材料进行了ZI-22和氨唑草酮的土壤淋溶性试验,结果表明:ZI-22较难淋溶,在田间条件下的半衰期为6.51 d,易降解,对环境和后茬作物较为安全;氨唑草酮易淋溶,但在田间条件下的半衰期为3.12 d,易降解,对环境和后茬作物也较为安全。ZI-22抗性风险评价。以莱茵衣藻为实验材料,用ZI-22和PDS抑制剂吡氟酰草胺驯化莱茵衣藻,经累代培养,最终得到抗性莱茵衣藻。结果表明:药剂驯化48代后,抗性莱茵衣藻对吡氟酰草胺和ZI-22的抗性倍数分别为3873倍和34.83倍,说明ZI-22的抗性风险极低。通过抗ZI-22及抗吡氟酰草胺莱茵衣藻交互抗性测定,结果表明抗ZI-22莱茵衣藻对吡氟酰草胺也同样具有极高的抗性,而抗吡氟酰草胺莱茵衣藻对ZI-22的抗性倍数为4.5倍,表明ZI-22与PDS抑制剂具有一定的交互抗性。通过本论文的研究,可知ZI-22在应用技术上具有以下特点:在玉米3-5叶期施药,ZI-22与氨唑草酮混剂推荐剂量为150 g a.i./hm~2;混剂在田间土壤中残留期短,可以进行二次施药,不会对后茬作物造成危害;ZI-22抗性风险低,可高频次使用,但与PDS除草剂存在一定的交互抗性,应避免长期与PDS除草剂交替使用。